Chick Embryo

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The chick embryo (Gallus gallus) pictured below was first soaked in methylene blue to stain the skeleton, then cleansed with ethanol, and finally immersed in an oil of wintergreen (methyl salicylate) bath to make the tissues transparent. The syringe needle, visible in the picture, provides scale.

Note the very large relative size of the eyes, which is characteristic of developing birds.

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