Perfect focus through thick layers

Better vision for medicine?

Theseus in the labyrinth killing the Minotaur Two different views of the same target. The first (a) was made using the conventional microscopy technique known as "two photon fluorescence." The second (b) is a far better resolved image made with the new waveform-shaping, guide-star-free technique developed by the researchers. The third (c) is a direct image of the target to verify the accuracy of the new technique.
microscope imaging
Artist's rendering of a shaped femtosecond pulse wavefront focusing through a scattering tissue. By pre-shaping the wave going into a complex scattering medium, it is possible to bring it to a sharp focus, enabling microscopy and other applications. Credit: Meital Covo

Sept. 11, 2014 — In medical and biological research, investigators often need to zoom in on diseased tissue or scan fragile biological samples. Such close focus often means the researcher is peering through layers of tissue and other materials that can blur and distort the image. Some modern microscopes can compensate for this image distortion, but only for weak aberrations or by using invasive "guide stars," imaging aids that provide a stable reference point.

In a first-of-its-kind demonstration, A team of researchers has developed a way to focus laser light through even the murkiest of surroundings without relying on a guide star. The demonstration was documented in a study published in The Optical Society's (OSA) new high-impact journal Optica. This innovation, a specialized version of an adaptive optics microscope, can resolve a point less than one thousandth of a millimeter across.

"Imagine shining a flashlight through a thick fog bank to try to see a single dot," said Yaron Silberberg, a researcher at the Weizmann Institute of Science in Israel and co-author on the Optica paper. "The light would become so scattered as it traveled through the fog that you wouldn't be able to make out what was hidden inside. By carefully shaping the light going in, however, it would be possible to home in on your target. That is exactly what the researchers were able to achieve in a way no one has ever done before."

microscope imaging Spatial Light Modulator (SLM) so they can pass through a scattering medium to image otherwise hidden details on a target sample. Credit: Optica

A standard two-photon scanning microscope uses bursts of laser light to build up a picture point-by-point and then line-by-line. The team modified the instrument by incorporating a high-resolution wavefront shaping deviceling the microscope to peer through a visually opaque obscuring layer that would otherwise produce a blurred, foggy image.

Wavefront shaping sees through the murk

The wavefront shaping approach is based on "adaptive optics," used in both science and medicine, to correct for the blurring of an image by analyzing the way light waves are distorted as they pass through different materials. In astronomy, for example, telescopes with adaptive optics remove the twinkling from starlight to show distant objects as clearly as if the telescope were in space. While adaptive optics works wonders to correct slightly distorted images, by such events as atmospheric turbulence, it cannot compensate for severe distortions, such as the scattering by fog or imaging through the shell of an egg.

Yet, recently, this new variation on the adaptive optics approach was shown to handle such tasks. Even when a perfect correction of the distortions is impossible, wavefront shaping can produce a crisp high-contrast image, even through visually opaque barriers.

Breaking free from the guide star

But wavefront shaping, like adaptive optics, requires a reference point to bring targets into sharp focus. This point is known as a guide star due to its first use in astronomy. It must be placed in relatively the same area or field of view as the object being studied. In astronomy, bright nearby stars or powerful lasers are used to adjust the optics of the telescope to produce a nearly perfect image.

In biology and medicine, however, guide stars could be an implanted fluorescent particle, and would need to be physically inserted into the imaged specimen. This process can interfere with the sample being studied or can damage delicate tissue. This is particularly problematic, for example, when trying to study a living embryo inside a shell.

"Until today, all-optical focusing through scattering media required invasively implanting a point-like guide star," said Ori Katz, a scientist at the Langevin Institute in Paris and co-author on the Optica paper. "For the first time, we have shown that it is possible to focus light through visually opaque barriers without using such a guide star."

To achieve this guide-star-free imaging, Silberberg and his colleagues used a standard laser-scanning two-photon microscope to focus in on a single point behind an obscuring scattering layer.

The laser emits light pulses lasting approximately 100 femtoseconds (a femtosecond is one millionth of a billionth of second), which are directed through the obscuring layer and onto a target. The microscope was able focus in on a point about one thousandth of a millimeter across.

As the light passes through the intervening layer, it becomes highly scattered. In conventional adaptive optics, the returning signal from the guide star would have been measured and then corrected or reshaped, into its original form. With no clear reference point or guide star, however, there would normally be no way of correcting such a highly scattered focus.

The researchers found the answer in the scattered light itself. Because two-photon fluorescence responds in a so-called nonlinear manner to the intensity of the excitation light, they were able to glean important information about the wavefront required to compensate for the scattering. Rather than the conventional adaptive optics approach, they altered the original pulsed light going in to form a focused beam that they later scanned to generate an image of the fluorescent object hidden behind the obscuring layer.

"What we have discovered is that it's possible to efficiently 'pre-correct' the laser beam using the nonlinear fluorescence signal," noted Katz. "The end result is that instead of having a distorted, blurred light source on the object to be imaged, we have a tightly focused, or in this case, refocused beam of light."

Very recently, other groups have shown that similar focusing is also possible using an acoustic-based guide star. But, according to the researchers, this combined optical/acoustical system is substantially more complicated and the focus is not nearly as sharp.

Future applications in imaging, surgery

The researchers also clarify that their technique is only a basic demonstration of the principle. More work is needed to put it to practical use. "We hope that it can help in microscopic imaging, such as in the direct imaging of embryonic development," said Silberberg. "It may also help in guiding laser surgery."

The next step in developing this technology is to shorten the amount of time it takes to achieve the necessary focus.

"We are excited about this project because it has produced new understanding and a new way of seeing through visually opaque samples," concluded Katz.

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